ELISA Kit for Interleukin 17 (IL17)

15.6-1, 000pg/mL

The minimum detectable dose of this kit is typically less than 5.5pg/mL

3h

This assay has high sensitivity and excellent specificity for detection of Interleukin 17 (IL17).
No significant cross-reactivity or interference between Interleukin 17 (IL17) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 17 (IL17). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 17 (IL17). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 17 (IL17), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 17 (IL17) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Function of interleukin?17 and ?35 in the blood of patients with hepatitis B?related liver cirrhosis; MicroRNA‐155 may be involved in the pathogenesis of atopic dermatitis by modulating the differentiation and function of T helper type 17 (Th18) cells; The immunomodulating effect of seminal plasma on T cells; Interleukin-17 levels correlate with poor prognosis and vascular endothelial growth factor concentration in the serum of patients with non-small cell lung cancer; Relationship between IL-17 serum level and ambulatory blood pressure in women with polycystic ovary syndrome; Relationship between IL-17 and ambulatory blood pressure in polycystic ovary syndrome; Low-density neutrophils in severe fever with thrombocytopenia syndrome (SFTS) display decreased function to phagocytose SFTS virus and enhanced capacity to synthesize cytokines; Increased Levels of IL-17, IL-23, MIP-1α, MCP-1 and Global Leukocytes in Fibromyalgia Patients; Lower level of IL‑35 and its reduced inhibition in Th17 cells in patients with bone marrow mononuclear cells Coombs test‑positive hemocytopenia.; Associations among interleukin-17, interferon-gamma, and acute coronary syndrome; Changes in Th1/Th2-producing cytokines during acute exacerbation chronic obstructive pulmonary disease; Seluang Fish (Rasbora Spp.) Oil Decreases Inflammatory Cytokines Via Increasing Vitamin D Level in Systemic Lupus Erythematosus; TRAF-6 调控TGF-β1-Smad2/Smad3 信号通路在Graves 病免疫发病机制中的作用;