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Human Interleukin 25, IL25 ELISA Kit

Human Interleukin 25, IL25 ELISA Kit

€2,815.00

Excl. VAT

Item no.	DEIA154
Manufacturer	Creative Diagnostics
Amount	10plates
Category	ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Specific against	Human (Homo sapiens)
Host	Human
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Available
Abbr	Human IL25 ELISA Kit
Full name	Interleukin 25
Storage	Capture Antibody: 100 ug ofantigen-affinity purified goat anti-IL-17E. Centrifuge vial prior to opening.Reconstitute in 1.0 ml water for a concentration of 100 ug/ml. Followingreconstitution the Capture antibodies may be stored at 2-8C for up to 6 months.For long term storage it is recommended to aliquot into working volumes andstore at -70C in amanual defrost freezer. Detection Antibody: 25 ug ofbiotinylated antigen-affinity purified goat anti-IL-17E. Centrifuge vialprior to opening. Reconstitute in 0.25 ml sterile water for a concentrationof 100 ug/ml. Following reconstitution the Detection antibodies may be storedat 2-8C for upto 6 months. For long term storage it is recommended to aliquot into workingvolumes and store at -70C in amanual defrost freezer. Human IL-17E Standard: 1 ug of recombinantIL-17E. Centrifuge vial prior to opening. Reconstitute in 1.0 ml sterile waterfor a concentration of 1 ug/ml. The Standard may be stored at 2-8C for one month oraliquoted and stored at -70Cfor up to three months in a manual defrost freezer. UltraAvidin-HRPConjugate: 40 ulvial. Upon receipt, UltraAvidin-HRP conjugateshould be stored at 2-8C, DONOT FREEZE. TMB Liquid Substrate: Aspirateand wash plate 4 times. Add 100 ul of TMB HRPO Microwell Substrate StandardKinetic One Component Ready Use to each well. Incubate at roomtemperature and monitorcolor development with an ELISA plate reader at 450 nmwith wavelength correction set at 540 nm or 570 nm. The reaction may bestopped after 15-20 minutes by adding 100 ul of 2 M sulfuric acid to eachwell.
Intended Use	RUO, HumanIL-17E ELISA development kit contains the key components required for thequantitativemeasurementof natural and/or recombinant IL-17E in a sandwich ELISA format within therange of 32-2, 000 pg/ml. Using the ELISA protocol described below, this kitprovides sufficient reagents to assay IL-17E in approximately 1, 000 ELISAplate wells.
Gene Information - Gene Name	IL25interleukin 25 [ Homo sapiens ]
Gene Information - Offical Symbol	IL25
Gene Information - Synonyms	IL25, interleukin25, interleukin 17E, IL17E, IL-25, IL-17E, Interleukin-25, UNQ3120/PRO10272, Interleukin-17E
Gene Information - GeneID	64806
Gene Information - mRNA Refseq	NM_022789
Gene Information - Protein Refseq	NP_073626
Gene Information - MIM	605658
Gene Information - UniProt ID	Q9H293
Gene Information - Function	cytokineactivity
ELISA Procedure	Standard/Sample: Dilutestandard from 2, 000 pg/ml to zero in diluent. Immediately add 100 ul ofstandard or sample to each well in duplicate and incubate at room temperaturefor at least 2 hours. Detection: Aspirateand wash plate 4 times. Dilute a portion of detection antibody in diluent toa concentration of 50 ng/ml. Add 100 ul per well and incubate at roomtemperature for 2 hours. UltraAvidin-HRPConjugate: Aspirate and wash plate 4 times. Dilute 3.0 ul ofavidin-HRP conjugate 1:5, 000 (this dilution factor may require someoptimization) in diluent for a total volume of 15 ml. Add 100ul per well andincubate at room temperature for 30 minutes. TMB Liquid Substrate:Add 100 ul of TMB HRPO Microwell Substrate StandardKinetic One Component Ready Use to each well. Incubate at roomtemperature for 20-30 minutes and monitor color development with an ELISAplate reader at 450 nm with wavelength correction set at 540 nm or 570 nm.Avoid placing plates in direct light. Stop Solution: The reactionmay be stopped after 15-20 minutes by adding 100 ul of 2 M sulfuric acid toeach well. All eye, hand, face, and clothing protection should be used whenworking with sulfuric acid.
Detection Method	Sandwich ELISA

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.