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Human Interleukin 5, IL5 ELISA Kit

Human Interleukin 5, IL5 ELISA Kit

€2,815.00

Excl. VAT

Item no.	DEIA115
Manufacturer	Creative Diagnostics
Amount	10plates
Category	Cell Biology Cell Culture Cytokines ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Specific against	Human (Homo sapiens)
Host	Human
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Available
Abbr	Human IL5 ELISA Kit
Full name	Interleukin 5
Storage	Capture Antibody: 25 ug of antigen-affinity purified goat anti-IL-5.Centrifuge vial prior to opening. Reconstitute in 0.25 ml sterile water for aconcentration of 100 ug/ml. Following reconstitution the Capture antibodiesmay be stored at 2-8C for up to 6 months. For long term storage it isrecommended to aliquot into working volumes and store at -70C in a manual defrost freezer. Detection Antibody: 50 ug ofbiotinylated antigen-affinity purified goat anti-IL-5. Centrifuge vial priorto opening. Reconstitute in 0.5 ml sterile water for a concentration of 100ug/ml. Following reconstitution the Detection antibodies may be stored at 2-8C for upto 6 months. For long term storage it is recommended to aliquot intoworking volumes and store at -70C in amanual defrost freezer. Human IL-5 Standard: 1 ug of recombinantIL-5. Centrifuge vial prior to opening. Reconstitute in 1.0 ml sterile waterfor a concentration of 1 ug/ml. The Standard may be stored at 2-8C for onemonth or aliquoted and stored at -70C for upto three months in a manual defrost freezer. UltraAvidin-HRP Conjugate: 40 ulvial. Upon receipt, UltraAvidin-HRP conjugate should be stored at 2-8C, DO NOT FREEZE. TMB Liquid Substrate: Aspirateand wash plate 4 times. Add 100 ul of TMB HRPO Microwell Substrate StandardKinetic One Component Ready Use to each well. Incubate at roomtemperature and monitorcolor development with an ELISA plate reader at 450 nmwith wavelength correction set at 540 nm or 570 nm. The reaction may bestopped after 15-20 minutes by adding 100 ul of 2 M sulfuric acid to eachwell.
Intended Use	RUO, HumanIL-5 ELISA development kit contains the key components required for thequantitative measurement of natural and/or recombinant IL-5 in a sandwichELISA format within the range of 32–2, 000 pg/ml. Using the ELISA protocoldescribed below, this kit provides sufficient reagents to assay IL-5 in approximately1, 000 ELISA plate wells.
Gene Information - Gene Name	IL5 interleukin 5 (colony-stimulating factor, eosinophil) [ Homo sapiens ]
Gene Information - Offical Symbol	IL5
Gene Information - Synonyms	IL5, interleukin 5 (colony-stimulating factor, eosinophil), EDF, IL-5, TRF, B celldifferentiation factor I, T-cell replacing factor, eosinophil differentiationfactor, interleukin 5, interleukin-5, B-cell differentiation factor I, Eosinophil differentiation factor, OTTHUMP00000065830
Gene Information - GeneID	3567
Gene Information - mRNA Refseq	NM_000879
Gene Information - Protein Refseq	NP_000870
Gene Information - MIM	147850
Gene Information - UniProt ID	P05113
Gene Information - Function	interleukin-5receptor activity, cytokine activity, growth factor activity
ELISA Procedure	Standard/Sample: Dilutestandard from 2, 000 pg/ml to zero in diluent. Immediately add 100 ul ofstandard or sample to each well in duplicate and incubate at room temperaturefor at least 2 hours. Detection: Aspirateand wash plate 4 times. Dilute a portion of detection antibody in diluent toa concentration of 50 ng/ml. Add 100 ul per well and incubate at room temperaturefor 2 hours. UltraAvidin-HRP Conjugate: Aspirate and wash plate 4 times. Dilute 3.0 ul of avidin-HRPconjugate 1:5, 000 (this dilution factor may require some optimization) indiluent for a total volume of 15 ml. Add 100ulper well and incubate at room temperature for 30 minutes. TMB Liquid Substrate:Add 100 ul of TMB HRPO Microwell Substrate StandardKinetic One Component Ready Use to each well. Incubate at roomtemperature for 20-30 minutes and monitor color development with an ELISAplate reader at 450 nm with wavelength correction set at 540 nm or 570 nm.Avoid placing plates in direct light. Stop Solution: The reactionmay be stopped after 15-20 minutes by adding 100 ul of 2 M sulfuric acid toeach well. All eye, hand, face, and clothing protection should be used when workingwith sulfuric acid.
Detection Method	Sandwich ELISA

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.