Comparison

MDBK (NBL-1) European Partner

€375.00
Excl. VAT
Item no. CLS-600396
Manufacturer CLS Cell Lines Service
Amount 1 cryovial
Category
Type Cell line
Specific against Cattle (Bovine)
Dry ice Yes
ECLASS 10.1 42040401
ECLASS 11.0 42040401
UNSPSC 41106509
Alias MDBK (NBL-1), NBL-1, Madin-Darby Bovine Kidney, Madin Darby Bovine Kidney
Available
Manufacturers Category
Bovine cell lines
Tissue
Kidney
Breed
Bos taurus
Age
Adult
Gender
Male
Morphology
Epithelial-like
Growth Properties
Monolayer, adherent
Biosafety Level
1
Viruses
The line was Tested and shown to be free of bovine diarrhoea virus.
Virus Susceptibility
The cells are susceptibled to bovine diarrhea virus, vesicular stomatitis (Indiana strain), infectious bovine rhinotracheitis virus, bovine parvovirus, bovine adenovirus I and III, and parainfluenza virus 3.
Virus Resistance
Poliovirus 2
Reverse Transcriptase
negative
Products
Keratin
Culture Medium
EMEM
Medium Supplements
10% FBS, 2 mM L-glutamine, 0.1 mM NEAA, 1 mM sodium pyruvate
Subculturing
Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Split Ratio
A ratio of 1:2 to 1:4 is recommended
Seeding Density
1 x 10^4 cells/cm^2
Fluid Renewal
Every 3 days
Freezing Recovery
Fast
Freeze Medium
NOTE
Deutsch:
Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen.
Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen.
Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt.

English:
University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase.
Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase.
After the order is received, all relevant documents will be sent to you.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 1 cryovial
Available: In stock
Listprice: €375.00
Price: €375.00
available

Delivery expected until 9/4/2025 

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