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NRK-EGFP2-Nup50

NRK-EGFP2-Nup50

€540.00

Excl. VAT

Item no.	CLS-500726
Manufacturer	CLS Cell Lines Service
Amount	1 cryovial
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	Rat (Rattus norvegicus)
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	NRK EGFP2-Nup50
Available
Manufacturers Category	NRK cells fluorescence tagged
Tissue	Kidney
Breed	OsborneMendel
Morphology	Fibroblast-like cells with fusiform shape
Growth Properties	Monolayer, adherent
Biosafety Level	1
Receptors Expressed	Epidermal growth factor (EGF), multiplication stimulating activity (MSA)
Protein Expression	EGFP3-Nup50
Products	NUP50 (Nucleoporin 50)
Culture Medium	DMEM
Medium Supplements	10% FBS, 2 mM L-glutamine, 4.5 g/L glucose, Add G418 to culture medium at a final concentration of 0.5 mg/ml
Subculturing	Remove medium and rinse with PBS. Add fresh 0.025% trypsin/0.02% EDTA solution at 37 ?C until cells detach (typically ~5 min). To remove trypsin, add fresh medium, transfer to a tube and centrifuge. Aspirate the supernatant, resuspend the cell pellet in culture medium and dispense into new flasks. Add G418 to culture medium at a final concentration of 0.5 mg/ml.
Split Ratio	A ratio of 1:3 to 1:4 is recommended
Seeding Density	2 to 4 x 10^4 cells/cm^2
Fluid Renewal	2 to 3 times per week
Freeze Medium	CM-1 (Catalog number CLS-800100)
NOTE	Deutsch: Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen. Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen. Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt. English: University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase. Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase. After the order is received, all relevant documents will be sent to you.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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CLS-500726-datasheet.pdf

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Amount: 1 cryovial

Listprice: €540.00

Price: €540.00

available

Delivery expected until 9/11/2025

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