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Human Mesenchymal Stem Cells, Bone Marrow (HMSC-BM)

Human Mesenchymal Stem Cells, Bone Marrow (HMSC-BM)

€690.00

Excl. VAT

Item no.	CLS-300665
Manufacturer	CLS Cell Lines Service
Amount	1 million cells
Category	Cell Lines & Cell Culture Cell Lines
Type	Cells
Specific against	Human (Homo sapiens)
Dry ice	Yes
ECLASS 10.1	32160890
ECLASS 11.0	32160890
UNSPSC	41106509
Available
Manufacturers Category	Mesenchymal Stem cells
Tissue	Bone Marrow
Applications	Drug testing, regenerative medicine, disease research
Age	Please inquire
Gender	Please inquire
Ethnicity	Caucasian
Morphology	Well-spread spindle shaped, fibroblast-like morphology for at least within 5 passages. Fewer than 2% cells exhibit spontaneous myofibroblast-like morphology within each passage.
Cell Type	Stem cell
Growth Properties	Adherent
Biosafety Level	1
Antigen Expression	A comprehensive panel of markers, including CD73/CD90/CD105 (positive) and CD14/CD34/CD45/HLA-DR (negative), are used in flow cytometry analysis to identify cultivated MSCs (P2-P3) prior to cryopreservation. These markers are recommended by the ISCT MSC committee.
Viruses	Donor is negative for HBV (PCR), Treponema pallidum (PCR), and HIV-1/2 (IFA). Cells are negative for HBV, HCV, HSV1, HSV2, CMV, EBV, HHV6, Toxoplasma gondii, Treponema pallidum, Chlamydia trachomatis, Ureaplasma urealyticum, and Ureaplasma parvum.
Culture Medium	Mesenchymal Cell Growth Medium X1
Passaging Solution	Trypsin-EDTA
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Seeding Density	1 to 3 x 10^3 cells/cm^2
Fluid Renewal	First fluid renewal after 24 hours, then every 2 to 3 days.
Freeze Medium	CM-1 (Catalog number CLS-800100)
NOTE	Deutsch: Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen. Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen. Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt. English: University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase. Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase. After the order is received, all relevant documents will be sent to you.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 million cells

Listprice: €690.00

Price: €690.00

available

Delivery expected until 9/4/2025

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