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SK-MEL-1

SK-MEL-1

€540.00

Excl. VAT

Item no.	CLS-300424
Manufacturer	CLS Cell Lines Service
Amount	1 cryovial
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	Human (Homo sapiens)
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	SK-Mel-1, SK Mel 1, SK-Mel 1, SK-Mel1, SKMEL-1, SkMEL-1, SKMEL1, SK 1
Available
Manufacturers Category	Skin cancer cell lines
Tissue	Skin
Disease	Melanoma
Metastatic Site	Thoracic lymph duct
Age	29 years
Gender	Male
Ethnicity	Caucasian
Morphology	Spherical
Growth Properties	Suspension
Biosafety Level	1
Antigen Expression	Blood Type A, Rh+. Antibody to this line was detected in 63% of patients with malignant melanoma and in 10% of patients with other diseases.
Isoenzymes	PGM3, 1, PGM1, 1, ES-D, 1, AK-1, 1, GLO-1, 1-2, G6PD, B,
Tumorigenic	Yes, in nude mice. Forms pigmented malignant melanomas. Also forms tumors in the cheek pouch of cortisone treated hamsters
Products	Melanin
Mutational Profile	V600E type BRAF Mutation was determined by DNA based methods (sequencing, RT-PCR) and protein based methods (Western Blot)
Culture Medium	EMEM
Medium Supplements	10% FBS, 2 mM L-glutamine
Subculturing	Cultures can be subcultured by addition or replacement of fresh medium. Establish new cultures at 1 x 10^5 viable cells/ml. Maintain at between 1 x 10^5 to 1 x 10^6 cells/ml.
Split Ratio	A ratio of 1:2 to 1:4 is recommended
Seeding Density	1 x 10^5 cells/mL
Fluid Renewal	2 to 3 times per week
Freeze Medium	CM-1 (Catalog number CLS-800100)
Amelogenin	X, Y
NOTE	Deutsch: Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen. Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen. Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt. English: University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase. Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase. After the order is received, all relevant documents will be sent to you.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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CLS-300424-datasheet.pdf

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Amount: 1 cryovial

Listprice: €540.00

Price: €540.00

available

Delivery expected until 9/4/2025

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