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LS-174T

LS-174T

€375.00

Excl. VAT

Item no.	CLS-300392
Manufacturer	CLS Cell Lines Service
Amount	1 cryovial
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	Human (Homo sapiens)
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	Ls174T, LS174t, Ls-174-T, LS-174-T, LS 174 T, LS174T, Ls-174T, LS 174T, LS-174, LS174
Available
Manufacturers Category	Intestine cancer cell lines
Tissue	Colon
Disease	Adenocarcinoma
Age	58 years
Gender	Female
Ethnicity	Caucasian
Morphology	Epithelial-like
Growth Properties	Adherent
Biosafety Level	1
Protein Expression	Colon Antigen 3 +, CEA +, p53 -, GFAP -, mRNA expression +
Antigen Expression	HLA A2, B13, B50, Blood type O
Isoenzymes	ADA, 1: G6PD, B, PGM1, 1, PGM3, 2, PGD, A, ES-D, 1, PEP-D, 1
Oncogenes	myc +, myb + , ras +, fos +, p53 +, sis -, abl -, ros -, src -
Tumorigenic	Yes, in nude mice
Reverse Transcriptase	negative
Products	Carcinoembryonic antigen (CEA) 1944 ng/106 cells in 10 days, mucin, interleukin-10 (IL-10), interleukin-6 (IL-6)
Mutational Profile	LS-174T cells carry a mutation in codon 12 of Kras gene: GGT(Wt Gly) \|BiggerAs\|GAT(Asp)
Karyotype	45, X with one X chromosome missing but no other chromosomal aberrations
Culture Medium	EMEM
Medium Supplements	10% FBS, 2 mM L-glutamin, 1% NEAA
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambiente temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Split Ratio	A ratio of 1:2 to 1:5 is recommended
Seeding Density	5 to 8 x 10^4 cells/cm^2
Fluid Renewal	2 to 3 times per week
Freezing Recovery	After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Freeze Medium	CM-1 (Catalog number CLS-800100)
Amelogenin	X, Y
NOTE	Deutsch: Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen. Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen. Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt. English: University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase. Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase. After the order is received, all relevant documents will be sent to you.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cryovial

Listprice: €375.00

Price: €375.00

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Delivery expected until 9/4/2025

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