SaOS-2

Bone cancer cell lines

Osteosarcoma

Female

Epithelial-like

1

Blood Type B, Rh+, HLA A2, A3, Bw16, Bw47

No

The stemline chromosome number is hypotriploid with the modal number of 56 chromosomes per cell and the 2S component occurring at 13.2%. Over two-thirds of the chromosome complement consisted of structurally rearranged chromosomes. Most marker chromosomes had complex rearrangements. The origin of the segments composing these markers could not be identified. Of the identifiable markers, 6p+/q+, 7p+, 11p+, and 12p+ occasionally were present at 2 copies per cell. The Y chromosome was not detected in the QM stained preparation.

5% FBS, 2 mM L-glutamine

Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypLETM (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.

1 x 10^4 cells/cm^2

Fast

Deutsch:
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Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen.
Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt.

English:
University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase.
Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase.
After the order is received, all relevant documents will be sent to you.