MCF-7

€375.00

Excl. VAT

Item no.	CLS-300273
Manufacturer	CLS Cell Lines Service
Amount	1 cryovial
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	Human (Homo sapiens)
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	MCF 7, MCF.7, MCF7, Michigan Cancer Foundation-7, ssMCF-7, ssMCF7, MCF7/WT, MCF7-CTRL, IBMF-7
Available
Manufacturers Category	Breast cancer cell lines
Tissue	Breast
Disease	Adenocarcinoma
Metastatic Site	Pleural effusion
Age	69 years
Gender	Female
Ethnicity	Caucasian
Morphology	Epithelial-like
Growth Properties	Monolayer, adherent
Biosafety Level	1
Receptors Expressed	The cells express the wildtype and variant estrogen receptors as well as progesterone receptor.
Protein Expression	p53 negative, pGP9.5 negative, CEA positive
Isoenzymes	PGM3, 1, PGM1, 1-2, ES-D, 1-2, AK-1, 1, GLO-1, 1-2, G6PD, B,
Oncogenes	wnt7h +, Tx-4
Tumorigenic	Yes, in nude mice
Products	Insulin-like growth factor binding proteins (IGFBP) BP-2, BP-4, BP-5
Mutational Profile	TP53 wt
Karyotype	The stemline chromosome numbers ranged from hypertriploidy to hypotetraploidy, with the 2S component occurring at 1%. There were 29 to 34 marker chromosomes per S metaphase, 24 to 28 markers occurred in at least 30% of cells, and generally one large submetacentric (M1) and 3 large subtelocentric (M2, M3, and M4) markers were recognizable in over 80% of metaphases. No DM were detected. Chromosome 20 was nullisomic and X was disomic. Phenotype Frequency Product: 0.0154
Culture Medium	EMEM
Medium Supplements	10% FBS, 2 mM L-glutamine
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypLE Express (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37 ?C for 10 to 15 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Split Ratio	A ratio of 1:3 to 1:6 is recommended
Seeding Density	3 x 10^4 cells/cm^2
Fluid Renewal	2 to 3 times per week
Freezing Recovery	Allow the cells to rest for 48 hours past thawing
Freeze Medium	CM-1 (Catalog number CLS-800650)
NOTE	Deutsch: Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen. Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen. Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt. English: University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase. Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase. After the order is received, all relevant documents will be sent to you.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cryovial

Listprice: €375.00

Price: €375.00

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Delivery expected until 9/4/2025

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