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Kasumi-1

Kasumi-1

€375.00

Excl. VAT

Item no.	CLS-300226
Manufacturer	CLS Cell Lines Service
Amount	1 cryovial
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	Human (Homo sapiens)
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	KASUMI-1, Kasumi 1, KASUMI1, Kasumi1
Available
Manufacturers Category	Leukemia cell lines
Tissue	Blood
Disease	Acute myeloblastic leukemia
Age	7 years
Gender	Male
Ethnicity	Japanese
Morphology	Round cells showing marked variations in both size and nuclear cytoplasmic ratio.
Cell Type	Myeloblast (AML-acute myeloid leukemia)
Growth Properties	Suspension
Biosafety Level	1
Antigen Expression	CD4+ (37.1%, coexpressed with CD34 and CD33), CD13+(OKM13), CD15+(LeuM1), CD33+, CD34+(MY10), CD38+(OKT10, 50.1%), CD71+(Nu-TERf), HLA-DR+(OKDR).
Karyotype	t(8, 21) chromosome translocation
Culture Medium	RPMI 1640
Medium Supplements	10% FBS, 2 mM L-glutamine
Doubling Time	40 to 45 hours
Subculturing	Start cultures at 3 x 10^5 cells/ml and split 24 hours later. Subculture the cells in transferring one part of cell suspension into new cell culture flasks already containing an appropriate volume of fresh cell culture medium. Maintain at a cell density between 3 x 10^5 and 6 x 10^5 cells/ml. Viability may drop when the cell density exceeds 1-2 x 10^6 cells/ml.
Split Ratio	A ratio of about 1:2 to 1:3 every 3 to 4 days is recommended
Seeding Density	1 x 10^5 cells/ml
Fluid Renewal	Add fresh medium (20 to 30% by volume) every 2 to 3 days
Freezing Recovery	About one week
Freeze Medium	CM-1 (Catalog number CLS-800650)
Amelogenin	X, X
NOTE	Deutsch: Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen. Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen. Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt. English: University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase. Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase. After the order is received, all relevant documents will be sent to you.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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CLS-300226-datasheet.pdf

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Amount: 1 cryovial

Listprice: €375.00

Price: €375.00

available

Delivery expected until 9/4/2025

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