HepG2

€540.00

Excl. VAT

Item no.	CLS-300198
Manufacturer	CLS Cell Lines Service
Amount	1 cryovial
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	Human (Homo sapiens)
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	HEP-G2, Hep G2, HEP G2, Hep-G2, HEPG2
Available
Manufacturers Category	Liver cancer cell lines
Tissue	Liver
Disease	Hepatocellular carcinoma
Applications	This cell line is an optimal choice for transfection. Further, the HepG2 cells offer an array of applications, ranging from 3D cell culture and cancer research to high-throughput screening and toxicology.
Age	15 years
Gender	Male
Ethnicity	Caucasian
Morphology	Epithelial-like
Growth Properties	Adherent
Biosafety Level	1
Receptors Expressed	insulin, insulin-like growth factor II (IGF II)
Protein Expression	p53 positive
Tumorigenic	no
Products	Albumin, alpha-fetoprotein (alpha fetoprotein), alpha1 acid glycoprotein (alpha-1 acid glycoprotein), alpha1 antitrypsin (alpha-1-antitrypsin), alpha1 antichymotrypsin, (alpha-1-antichymotrypsin), alpha2 HS glycoprotein (alpha-2-HS- glycoprotein), alpha2 macroglobulin (alpha-2-macroglobulin), beta lipoprotein (beta-lipoprotein), ceruloplasmin, C4 and C3 activator, fibrinogen, haptoglobin, plasminogen, retinol binding protein (retinolbinding protein), transferrin
Karyotype	modal number = 55 (range = 50 to 60), has a rearranged chromosome 1
Culture Medium	Hams F12
Medium Supplements	10% FBS, 2 mM L-glutamine
Doubling Time	48 hours
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Split Ratio	A ratio of 1:4 to 1:6 is recommended
Seeding Density	2 to 3 x 10^4 cells/cm^2 during routine culture
Fluid Renewal	2 to 3 times per week
Freezing Recovery	Start culture using the complete contents of the cryovial in 2xT25 cell culture flasks. The cells will recover within 48 to 72 hours.
Freeze Medium	CM-1 (Catalog number CLS-800650)
Amelogenin	X, Y
NOTE	Deutsch: Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen. Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen. Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt. English: University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase. Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase. After the order is received, all relevant documents will be sent to you.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

Request product datasheet

Request safety datasheet

Simple Order Order Subscription

Amount: 1 cryovial

Listprice: €540.00

Price: €540.00

available

Delivery expected until 9/4/2025

Quantity:

Compare