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NCI-H69 (H69)

NCI-H69 (H69)

€375.00

Excl. VAT

Item no.	CLS-300185
Manufacturer	CLS Cell Lines Service
Amount	1 cryovial
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	Human (Homo sapiens)
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	NCI-H-69, NCI H69, H69, H-69, NCIH69, NCI-HUT-69, H69/P, NCI-H69C, H69C, H69c
Available
Manufacturers Category	Lung cancer cell lines
Tissue	Lung
Disease	Lung small cell carcinoma
Metastatic Site	Pleural effusion
Age	55 years
Gender	Male
Ethnicity	Caucasian
Growth Properties	Floating aggregates
Biosafety Level	1
Receptors Expressed	Insulin-like growth factor II receptor (IGF II)
Protein Expression	p53 negative, cytokeratins positive
Isoenzymes	G6PD, B, PGM1, 2, PGM3, 1, ES-D, 2, Me-2, 1, AK-1, 1, GLO-1, 1-2, Phenotype Frequency Product: 0.00006
Tumorigenic	Forms tumors with typical small cell carcinoma histology
Karyotype	Aneuploid, with 3p deletion. Range = 40 to 73
Culture Medium	RPMI 1640
Medium Supplements	10% FBS, 2 mM L-glutamine, 4.5 g/L glucose
Doubling Time	69 hours
Subculturing	Allow aggregates to settle to the bottom of the flask, remove and discard the supernatant medium. Add fresh medium, disperse cells by gentle pipetting and dispense into new flasks. Subculture every 6 to 8 days.
Split Ratio	A ratio of 1:2 to 1:4 is recommended
Seeding Density	1 x 10^5 cells/mL
Fluid Renewal	2 to 3 times per week
Freezing Recovery	After thawing allow the cells to recover from the freezing process for at least 24 hours.
Freeze Medium	CM-1 (Catalog number CLS-800100)
NOTE	Deutsch: Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen. Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen. Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt. English: University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase. Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase. After the order is received, all relevant documents will be sent to you.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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CLS-300185-datasheet.pdf

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Amount: 1 cryovial

Listprice: €375.00

Price: €375.00

available

Delivery expected until 9/11/2025

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