DMS-79

€375.00

Excl. VAT

Item no.	CLS-300164
Manufacturer	CLS Cell Lines Service
Amount	1 cryovial
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	Human (Homo sapiens)
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	DMS 79, DMS79
Available
Manufacturers Category	Lung cancer cell lines
Tissue	Lung
Disease	Carcinoma, azaserine induced
Metastatic Site	Pleural effusion
Age	65 years
Gender	Male
Ethnicity	Caucasian
Growth Properties	Aggregates in suspension
Biosafety Level	1
Receptors Expressed	Epidermal growth factor (EGF)
Antigen Expression	Leu 7, My23, Class 1 HLA, Class 2 HLA
Oncogenes	c-myc +, N-myc +, c-raf-1 +, Ha-ras +, Ki-ras +, N-ras +, v-fes -, v-fms -
Tumorigenic	Yes, in nude mice
Products	Adrenocorticotropin (adrenocorticotropic hormone, ACTH), bombesin, calcitonin, corticotropin, beta endorphin, 17 beta estradiol, lipotropin, oxytocin - neurophysin (OT-NP), parathormone, somatostatin-like immunoreactivity (SRIF)
Culture Medium	RPMI 1640
Medium Supplements	10% FBS, 2 mM L-glutamine, 4.5 g/L glucose
Doubling Time	96 hours
Subculturing	Once or twice a week add 5 ml of fresh cell culture medium, as soon as the culture medium gets acidic. Subculture as soon as many very large clusters are observed. Dissociate the clusters by collecting the cells, rinsing once using PBS without calcium/magnesium and adding 3-5 ml Accutase. Incubate at 37 ?C for 10minutes. Collect the cells following centrifugation, resuspend in fresh cell culture medium and count. Start cultures at 2-4 x 10^4 cells/ml.
Split Ratio	A ratio of 1:2 to 1:4 is recommended
Seeding Density	2 to 4 x 10^4 cells/cm^2
Fluid Renewal	2 to 3 times per week
Freezing Recovery	After thawing, allow the cells to recover from the freezing process for at least 24 hours.
Freeze Medium	CM-1 (Catalog number CLS-800100)
Amelogenin	X, Y
NOTE	Deutsch: Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen. Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen. Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt. English: University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase. Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase. After the order is received, all relevant documents will be sent to you.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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CLS-300164-datasheet.pdf

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Amount: 1 cryovial

Listprice: €375.00

Price: €375.00

available

Delivery expected until 9/4/2025

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