Calu-1

€375.00

Excl. VAT

Item no.	CLS-300141
Manufacturer	CLS Cell Lines Service
Amount	1 cryovial
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	Human (Homo sapiens)
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	CaLu-1, CALU-1, Calu.1, CALU 1, Calu 1, CALU1, Calu1
Available
Manufacturers Category	Lung cancer cell lines
Tissue	Lung
Disease	Carcinoma
Metastatic Site	Pleural effusion
Age	47 years
Gender	Male
Morphology	Epithelial-like
Cell Type	Epidermoid
Growth Properties	Adherent
Biosafety Level	1
Protein Expression	p53 negative
Antigen Expression	Blood Type A, Rh+, HLA A10, A11, B15, Bw35
Isoenzymes	Me-2, 1-2, PGM3, 1, PGM1, 1-2, ES-D, 1, AK-1, 1, GLO-1, 1-2, G6PD, B, Phenotype Frequency Product: 0.0359
Oncogenes	K-ras oncogene positive.
Karyotype	The stem line chromosome number is hypotriploid and the 2S component occurred at 14.2%. Modal chromosome number is 62. Seven markers occurred frequently, M1 (two copies per cell), M6 and M7 were found in most cells, M2 and M3, and M4 and M5 appeared to be mutually exclusive, i.e., only one of M2 or M3, and one of M4 or M5 were present in each cell. Y chromosome was not detected by QM band examination, although the cell line was initiated from a male.
Culture Medium	EMEM
Medium Supplements	10% FBS, 2 mM L-glutamine, 1 mM sodium pyruvate
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Split Ratio	A ratio of 1:2 to 1:4 is recommended
Seeding Density	1 x 10^4 cells/cm^2 will result in a 90% confluent monolayer in about 4 days
Fluid Renewal	2 to 3 times per week
Freezing Recovery	After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Freeze Medium	CM-1 (Catalog number CLS-800650)
Amelogenin	X, X
NOTE	Deutsch: Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen. Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen. Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt. English: University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase. Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase. After the order is received, all relevant documents will be sent to you.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cryovial

Listprice: €375.00

Price: €375.00

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Delivery expected until 9/4/2025

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