BT-474

€375.00

Excl. VAT

Item no.	CLS-300131
Manufacturer	CLS Cell Lines Service
Amount	1 cryovial
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	Human (Homo sapiens)
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	Bt-474, BT474
Available
Manufacturers Category	Breast cancer cell lines
Tissue	Breast, mammary gland
Disease	Invasive ductal carcinoma
Metastatic Site	Ductal
Age	60 years
Gender	Female
Ethnicity	Caucasian
Morphology	Epithelial-like
Growth Properties	The cells grow in compact, slowly growing multi-layered colonies which rarely become confluent. A confluent monolayer is not formed.
Biosafety Level	1
Receptors Expressed	HER-2/NEU+, ER+, PR+
Isoenzymes	G6PD, B, PGM3, 1, PGM1, 1, ES-D, 1, Me-2, 0, AK-1, 1, GLO-1, 1, Phenotype Frequency Product: 0.0426
Tumorigenic	Yes, in nude mice
Virus Susceptibility	mouse mammary tumor virus (RIII-MuMTV)
Msi Status	Stable (MSS)
Mutational Profile	TP53 mut
Karyotype	mode = 55, range = 50 to 112, bimodal shift 58 - 59 and 100 in later passages with 3 marker chromosomes
Culture Medium	DMEM:Hams F12
Medium Supplements	5% FBS, 2 mM L-glutamine
Doubling Time	60 to 80 hours
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Split Ratio	A ratio of 1:2 to 1:3 is recommended
Seeding Density	2 x 10^4 cells/cm^2 will yield in a mostly confluent layer in about 4 days
Fluid Renewal	2 to 3 times per week
Freezing Recovery	Almost 100% recovered cells at \|BiggerAs\|90% viability
Freeze Medium	CM-1 (Catalog number CLS-800650)
Amelogenin	X, X
NOTE	Deutsch: Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen. Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen. Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt. English: University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase. Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase. After the order is received, all relevant documents will be sent to you.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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CLS-300131-datasheet.pdf

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Amount: 1 cryovial

Listprice: €375.00

Price: €375.00

available

Delivery expected until 8/21/2025

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