AN3 Ca

Reproductive system cancer cell lines

Adenocarcinoma

Female

Epithelial-like

Adherent

PGM3, 1-2, PGM1, 1, ES-D, 1, AK-1, 1-2, GLO-1, 2, G6PD, B,

Aneuploid, Phenotype Frequency Product: 0.0054

10% FBS, 2 mM L-glutamine

Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. Using collagen-coated flasks increases the number of adherent cells.

An initial seeding density of 3 to 4 x 10^4 cells/cm^2 is recommended. Later on, 2 x 10^4 cells/ cm^2 will yield a confluent layer in 4 to 5 days.

Within 24 to 48 hours

X, X