Monkey IL-4 Matched Antibody Pair for ELISPOT

Specificity: This kit is cross-reactive with rhesus macaque, cynomolgus, baboon and pig-tailed macaque Coating antibodies (2 vials) Product: Monoclonal antibody to monkey interleukin 4 (IL-4) Isotype: Mouse IgG1 Production: In vitro using serum free medium Purification: Ion exchange chromatography Contents: Each vial contains sufficient material for coating of five 96-well ELISPOT plates Buffer: Prior to lyophilization: 0.25 ml PBS + 125 mM trehalose Application: Coating antibody in an ELISPOT system Reconstitution: Dissolve the contents of one vial by injection of 0.25 ml distilled water into the vial and dilute 100 times in PBS. The total amount of one vial is sufficient for five 96-well ELISPOT plates (480 determinations, 50 ul/well). Detector antibodies (2 vials) Product: Biotinylated monoclonal antibody to monkey interleukin 4 (IL-4) Isotype: Rat IgG1 Production: In vitro using serum free medium Purification: Protein G-affinity chromatography Labeling: With Biotin-7-NHS (N-hydroxysuccinimide) Contents: Each vial contains sufficient material for five 96-well ELISPOT plates Buffer: Prior to lyophilization: 0.5 ml PBS + 1% BSA + 125 mM trehalose Application: Detection antibody in an ELISPOT system Reconstitution: Dissolve the contents of one vial by injection of 0.5 ml distilled water into the vial and dilute 100 times in Dilution buffer. The total amount of one vial is sufficient for five 96-well ELISPOT plates (480 determinations, 100 ul/well). General Specificity: Validated for detecting rhesus macaque, cynomolgus monkey, pig-tailed macaque, Japanese macaque, crested black macaque, barbary macaque, lion-tailed macaque, baboon, mandrill, black mangabey and Hanuman langur IL-4 Sterility: Membrane filtered (0.2 um) Stability: The lyophilized products are stable for more than one year at 4C. After reconstitution, the antibodies are stable for several months at 4C (if kept sterile) or for minimal one year at –20C.