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Anti-Mouse CD3 Purified Size 25 ug

Anti-Mouse CD3 Purified Size 25 ug

€65.00

Excl. VAT

Item no.	05112-20-25
Manufacturer	BioGems
Amount	25 ug
Category	Antibodies Flow Cytometry Immunohistochemistry Immunoprecipitation Western Blot Immunofluorescence Primary Antibodies
Type	Antibody
Format	Purified
Applications	WB, FC, IF, IP, IHC
Clone	17A2
Specific against	Mouse (Murine, Mus musculus)
Host	Hamster
Isotype	IgG
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Available
Description	The 145-2C11 monoclonal antibody reacts with mouse CD3e, the 20 kDa ε chain of the TCR complex. Together with the γ and δ subunits of CD3, the ε subunits are involved in the assembly, trafficking, and surface expression of T-cell receptor complex. CD3 is expressed on thymocytes, mature T cells, and natural killer T cells, and the ε chain enhances the antigen recognition. The 145-2C11 antibody binds to the TCR complex and, depending on the conditions, initiates T cells activation, proliferation, and apoptosis. The soluble antibody seems to block lysis of target cells by antigen-specific cytotoxic T lymphocytes. The 145-2C11 antibody does not cross-react with the rat leukocytes, and it is used as a phenotypic marker for mouse T lymphocytes.
Research Interest	Adaptive Immunity
Preperation	The product should be stored undiluted at 4C. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µ g of the protein.
Formulation	Phosphate-buffered aqueous solution, ph7.2.
References	Leo, O., Foo, M., Sachs, D. H., Samelson, L. E., & Bluestone, J. A. (1987). Identification of a monoclonal antibody specific for a murine T3 polypeptide.Proceedings of the National Academy of Sciences, 84(5), 1374-1378. Nakano, H., Yamazaki, T., Miyatake, S., Nozaki, N., Kikuchi, A., & Saito, T. (1996). Specific Interaction of Topoisomerase II and the CD3 Chain of the T Cell Receptor Complex. Journal of Biological Chemistry, 271(11), 6483-6489. Salmond, R. J., Filby, A., Pirinen, N., Magee, A. I., & Zamoyska, R. (2011). Mislocalization of Lck impairs thymocyte differentiation and can promote development of thymomas. Blood, 117(1), 108-117.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 25 ug

Listprice: €65.00

Price: €65.00

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Delivery expected until 9/11/2025

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