Anti-Human CD64 (FCGR1) - Purified In vivo PLATINUM Functional Grade

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Mouse

Immunogen

Rheumatoid synovial fluid cells and fibronectin purified human monocytes.

Product Concentration

≥ 5.0 mg/ml

Endotoxin Level

≤ 0.5 EU/mg as determined by the LAL method

Purity

≥98% monomer by analytical SEC

⋅

> 95% by SDS Page

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Storage and Handling

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

RRID

AB_2893829

Applications and Recommended Usage?
Quality Tested by Leinco

FC The suggested concentration for this CD64 antibody, clone 10.1, for staining cells in flow cytometry is ≤ 1.0 μg per 10⁶ cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.

Other Applications Reported In Literature ?

B
IHC FF
FA

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone 10.1 recognizes the alpha subunit of human FCGR1.

Antigen Distribution

FCGR1 is expressed on monocytes, macrophages, dendritic cells (DCs), and activated granulocytes.

Background

FCGR1 antibody, 10.1, recognizes high-affinity immunoglobulin gamma Fc receptor I (FCGR1), also known as CD64. FCGR1 is a 72 kDa type I transmembrane glycoprotein expressed on monocytes, macrophages, and dendritic cells (DCs). FCGR1 can also be induced on neutrophils with IFNγ and G-CSF¹. FCGR1 binds with high affinity to monomeric IgG1 and IgG3, and to a lesser extent, IgG4², resulting in phosphorylation of the intracellular FCGR1 ITAM motif and subsequent recruitment of Syk. FCGR1 contributes to inflammation via several mechanisms, including promoting antibody-dependent cell-mediated cytotoxicity (ADCC), clearance of immune complexes, cytokine production, and antigen presentation^{1, 3}. CD64-based targeted therapies eliminate M1 pro-inflammatory macrophages and show clinical potential for the treatment of macrophage-mediated chronic inflammatory diseases, such as chronic cutaneous inflammation and rheumatoid arthritis⁴. In addition, CD64 promotes antitumor responses and mediates cytotoxic killing of tumor cells by macrophages⁵.

Antigen Details

Protein

CD64

Ligand/Receptor

IgG receptor

PubMed

CD64

NCBI Gene Bank ID

2209

UniProt.org

UniProtKB - P12314 (FCGR1_HUMAN)

Research Area

Immunology

.

Innate Immunity

References & Citations

1. Hulett MD & Hogarth PM. (1998) Mol Immunol. 35(14-15):989-96

2. M. Daëron., et al. (2009) Blood. 113: 3716–3725

3. Alter G., et al. (2011) Epub. 415(2):160-7

4. Barth S., et al. (2017) Biomedicines. 5(3):56

5. Keler T., et al. (1998) Clin Cancer Res. 4(9):2237-43

Technical Protocols