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Anti-Caspase 9/CASP9 Antibody

Anti-Caspase 9/CASP9 Antibody

€366.00

Excl. VAT

Item no.	BOS-PA1595
Manufacturer	Boster
Amount	100 ug/vial
Category	Oncology Neuroscience Neural Signaling Neural Epigenetics Protein Aggregation, Clearance & Misfolding Assisted reproductive technologies Embryo manipulation Antibodies Cell Biology Cell Status Apoptosis Immunohistochemistry Western Blot Cardiovascular Research Ischemia & Reperfusion Injury Primary Antibodies By Organ Colorectal Cancer Heart & Blood Cancer Testicular & Prostate Cancer Gastric Cancer Uterine, Ovarian & Cervical Cancer
Type	Antibody Polyclonal
Format	Lyophilized
Applications	WB, IHC
Specific against	Human (Homo sapiens)
Host	Rabbit
Isotype	IgG
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Caspase-9;CASP-9;3.4.22.62;Apoptotic protease Mch-6;Apoptotic protease-activating factor 3;APAF-3;ICE-like apoptotic protease 6;ICE-LAP6;Caspase-9 subunit p35;Caspase-9 subunit p10;CASP9;MCH6;
Similar products	Apoptosis related cysteine peptidase antibody, APAF 3 antibody, APAF-3 antibody, APAF3 antibody, Apoptotic protease activating factor 3 antibody, Apoptotic protease Mch 6 antibody, Apoptotic protease Mch-6 antibody, Apoptotic protease-activating factor 3 antibody, CASP 9 antibody, CASP-9 antibody, CASP9 antibody, CASP9_HUMAN antibody, Caspase 9 apoptosis related cysteine peptidase antibody, Caspase 9 Dominant Negative antibody, Caspase 9c antibody, Caspase-9 antibody, Caspase-9 subunit p10 antibody, Caspase9 antibody, ICE LAP6 antibody, ICE like apoptotic protease 6 antibody, ICE-LAP6 antibody, ICE-like apoptotic protease 6 antibody, MCH6 antibody, RNCASP9 antibody
Available
Manufacturers Product Category	Primary Antibodies, Rabbit Polyclonal Antibodies
Manufacturers Research Category	Apoptosis, Apoptotic Markers, Cancer, Caspases, Cell Biology, Cell Death, Intracellular, Invasion/Microenvironment, Metabolism, Metabolism Processes, Pathways and Processes
Short Description	Boster Bio Anti-Caspase 9/CASP9 Antibody catalog # PA1595. Tested in IHC, WB applications. This antibody reacts with Human.
Description	Boster Bio Anti-Caspase 9/CASP9 Antibody catalog # PA1595. Tested in IHC, WB applications. This antibody reacts with Human.
Background	CASP9 (CASPASE9), also called APAF3, is an initiator caspase, encoded by the CASP9 gene. The CASP9 gene is mapped to chromosome 1p36.3-p36.1 by FISH. CASP9 is identified as a member of the caspase family that participates in caspase-3 activation in vitro. And it also regarded as the most upstream member of the apoptotic protease cascade that is triggered by cytochrome c and dATP. Its genomic coordinates (GRCh37) is 1:15, 818, 768-15, 851, 284. The crystal structure of CASP9 is complex with the BIR3 in an inhibitory domain of XIAP at 2.4-angstrom resolution and the CASP9 gene contains 9 exons and spans approximately 35 kb of genomic DNA. Caspase-9 and APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cytochrome c and dATP, an event that leads to caspase-9 activation. CASP9 activity increases dramatically upon association with the apoptosome complex. And the majority of Casp9 knockout mice died perinatally with a markedly enlarged and malformed cerebrum caused by reduced apoptosis during brain development.
Gene Name	CASP9
Application Details	Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Western blot, 0.1-0.5μg/ml, Human
Application Notes	Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
Clonality	Polyclonal
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Contents	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen	A synthetic peptide corresponding to a sequence in the middle region of human Caspase-9.
Purification	Immunogen affinity purified.
Cross-reactivity	No cross-reactivity with other proteins
Storage	Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Gene Full Name	Caspase-9
Molecular Weight	46281 MW
Protein Function	Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf- 1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly (ADP- ribose) polymerase (PARP).
Tissue Specificity	Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes..
Protein Name	Caspase-9(CASP-9)
Sequence Similarities	Belongs to the peptidase C14A family.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.