Comparison

Vimentin (L79) polyclonal antibody

€262.00
Excl. VAT
Item no. BS1855-50
Manufacturer Bioworld Technology, Inc
Amount 50ul
Category
Type Antibody Polyclonal
Applications WB, IF, IHC
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Vimentin, VIM
Similar products Vimentin
Available
Swiss-Prot
P08670
Application all
WB: 1:500ca.1:1000
IHC: 1:50ca.1:200
IF: 1:50ca.1:200
Background
Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. Intermediate filaments are constructed from two-chain, alpha-helical, coiled-coil molecules arranged on an imperfect helical lattice and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. One such intermediate filament protein, Vimentin, is a general marker of cells originating in the mesenchyme. Vimentin is frequently coexpressed with other members of the intermediate filament family, such as the cytokeratins, in neoplasms including melanoma and breast carcinoma.
Product
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Purification and Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Specificity
Vimentin (L79) pAb detects endogenous levels of Vimentin protein.
WB
Western blot (WB) analysis of Vimentin (L79) pAb at 1:500 dilution
Lane1:HEK293T whole cell lysate
Lane2:Raw264.7 whole cell lysate
Lane3:H9C2 whole cell lysate
BiowMW
ca. 40, 57 kDa
Note
For research use only, not for use in diagnostic procedure.
Alternative Name
Vimentin, VIM
Immunogen
Synthetic peptide, corresponding to amino acids 31-80 of Human Vimentin.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 50ul
Available: In stock
Listprice: €262.00
Price: €262.00
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