Synaptophysin (L128) polyclonal antibody

P08247

Synaptic vesicles participate in a cycle of fusion with the plasma membrane and reformation by endocytosis. Synaptic vesicle protein synaptophysin (SYP) is targeted to early endosomes in transfected fibroblasts and in neuroendocrine cells. SYP is an N-glycosylated intergral membrane protein found in neurons and endocrine cells that associates into hexamers to form a large conductance channel. SYP contains four transmembrane domains and may function as a gap juction-like channel. Membrane cholesterol specfically interacts with SYP to play a role in vesicle formation. Synaptobrevin (VAMP) also binds to SYP and the resultant complex is upregulated during neuronal development, but is absent in exocytosis fusion complex. Thus, the synaptophysin-synaptobrevin complex is not essential for exocytosis, but rather provides a pool of synaptobrevin for exocytosis.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Synaptophysin (L128) pAb detects endogenous levels of Synaptophysin protein.

Immunohistochemistry (IHC) analyzes of Synaptophysin (L128) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

For research use only, not for use in diagnostic procedure.

Synthetic peptide, corresponding to amino acids 101-150 of Human Synaptophysin.