GTF2F2 polyclonal antibody

P13984

In eukaryotic systems, initiation of transcription from protein-coding genes is a complex process requiring RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH, and sequence-specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. TFIIF, a heteromer composed of a small (RAP 30) and a large (RAP 74) subunit, is required for RNA polymerase II to assemble into a preinitiation complex formed by promotor DNA and the general factors TFIID, IIA and IIB. In addition, TFIIF stimulates transcription elongation by RNA polymerase II.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

GTF2F2 pAb detects endogenous levels of GTF2F2 protein.

Immunofluorescence analysis of A549 cells, using GTF2F2 polyclonal antibody

For research use only, not for use in diagnostic procedure.

Recombinant full length Human GTF2F2.