DAPI, 10mg/mL in H2O

DAPI (4', 6-Diamidino-2-Phenylindole) is a popular blue DNA dye that is used as a nuclear counterstain in fluorescence microscopy, chromosome staining and flow cytometry. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement, with higher affinity for A-T rich regions. Because of the potential toxicity of DAPI, we offer DAPI dilactate dissolved in H₂O as a safer alternative to weighing out the powdery form. We also offer DAPI dilactate solid, which is more water soluble than the dihydrochloride form of the dye.

• Classic blue nuclear counterstain for fixed cells
• Can be used at higher concentrations to stain live cells
• &lamba; _Ex/&lamba; _Em (with DNA) = 358/461 nm
• Yellow solution
• Store at 4C and protect from light
• C₂₂H₂₇N₅O₆
• MW: 457.49