NucView 488 and MitoView 633 Apoptosis Assay Kit (100 assays)

This kit contains the NucView® 488 Caspase-3 Substrate and the MitoView™ 633 mitochondrial dye. The kit provides a convenient tool for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using either fluorescence microscopy or flow cytometry. The bi-functional NucView® 488 Caspase-3 Substrate is able to detect both intracellular caspase-3 and at the same time stain the cell nucleus, which is known to undergo morphological changes during apoptosis. MitoView™ 633 is a far-red fluorescent mitochondrial dye. The staining is dependent upon the mitochondrial membrane potential; thus, apoptotic cells exhibit a much lower MitoView™ 633 dye staining compared to healthy cells. Furthermore, the spectral separation of these two dyes minimizes fluorescence overlap. Note: The optimal detection settings for MitoView™ 633 are the same as for Cy® 5 and other far-red dyes. However, the dye also has visible red fluorescence and can be imaged using Cy® 3 settings as well. As a consequence, the dye cannot be used for two-color imaging with other red probes. See our full selection of NucView® substrates and kits. We also offer MitoView™ Dyes separately. NucView® enzyme substrate technology is covered by U.S. Patents. Cy dye is a registered trademark of GE Healthcare.