TMB ONE Accessory Reagent (OOSA09597)

Liquid Ready to use TMB solution

OOSA09597 is a high performance TMB (3, 3A´, 5, 5A´- tetramethylbenzidine) solution, recommended for use in ELISA detection systems as a substrate for horseradish peroxidase (HRP). TMB CORE contains TMB, substrate buffer and hydrogen peroxide in a safe, ready to use solution. OOSA09597 produces a deep blue colour during the enzymatic degradation of H2O2 by horseradish peroxidase. For kinetic assays the absorbance is read at 655nm (blue). However, for endpoint assays the reaction may be stopped with 0.2M sulphuric acid, resulting in a yellow colour read at 450nm.