Chicken Anti-FLAG® Epitope Antibody 100ug

Anti-DYKDDDDK Epitope Tag. Chickens were immunized with a synthetic peptide containing the DYKDDDDK sequence conjugated to keyhole limpet hemocyanin (KLH). The sequence of this peptide corresponds to the FLAG®, epitope tag. After repeated injections, immune eggs were collected from the hens, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity purified using an agarose matrix to which the cognate peptide was attached. Eluates of this column were dialyzed against PBS, had their concentrations adjusted to 100 ug/ml, and then filter-sterilized.

Room Temperature