Actin Chicken Polyclonal Antibody

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (PBS, pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Actin Antibody (for nitrocellulose membrane loading control studies). Chickens were immunized with purified actin protein. After repeated injections, immune eggs were collected from the hens, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using an agarose matrix to which the whole protein was attached. The final preparation of this product involved mixing affinity purified anti-actin antibody (final concentration of 25 ug/ml) with IgY fractions of anti-actin antibody (final concentration of 10 mg/ml) in PBS with 50% glycerol. This preparation was then filter-sterilized.

1:2500-1:5000 Western Blot.

Room Temperature

0.4 ml, 10.0 mg/ml