Anti-Mouse IFNgamma (Clone XMG1.2) - Purified In vivo GOLD Functional Grade

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Rat

Immunogen

Purified Recombinant Mouse IFN-γ (> 98%)

Product Concentration

≥ 5.0 mg/ml

Endotoxin Level

≤ 1.0 EU/mg as determined by the LAL method

Purity

≥95% monomer by analytical SEC

⋅

> 95% by SDS Page

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Storage and Handling

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

2-8°C

RRID

AB_2737526

Applications and Recommended Usage?
Quality Tested by Leinco

ELISA Cap XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA with a concentration range of 0.5-2.0 µg/ml as recommended.
WB The suggested concentration for this XMG1.2 antibody for use in western blotting is 1-10 μg/ml.

Other Applications Reported In Literature ?

ICFC
ELISPOT Cap
ELISPOT Det
CyTOF®
N

Additional Reported Applications For Relevant Conjugates ?

IHC FF
WB
For specific conjugates of this clone, review literature for suggested application details.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rat Anti-Mouse Interferon Gamma (IFN-γ) (Clone XMG1.2) recognizes an epitope on Mouse IFN-γ. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

Interferon-gamma (IFN-γ) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons.¹ It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.² IFN-γ has antiviral, immunoregulatory, and anti-tumour properties.³

Antigen Details

Protein

IFN-γ

PubMed

IFN-γ

NCBI Gene Bank ID

15978

UniProt.org

Information on Uniprot.org

Research Area

Cell Biology

.

Immunology

.

Neuroinflammation

.

Neuroscience

References & Citations

1). Goeddel DV et al. (1982) Nature 298: 859
2.) Wilson CB et al. (2007) Adv. Immunol. 96: 41
3.) Hume DA et al. (2004) J Leukoc Biol. 75: 163
4.) Teijaro et al. (2020) bioRxiv 22: 24
5.) Hawman DW, et al. (2021) Microorganisms 9(2):279 Journal Link

Technical Protocols