Anti-Mouse CD54 (Clone YN1/1.7.4) - Purified In vivo PLATINUM Functional Grade

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Rat

Immunogen

Mouse NS-1 cells

Product Concentration

≥ 5.0 mg/ml

Endotoxin Level

< 0.5 EU/mg as determined by the LAL method

Purity

≥98% monomer by analytical SEC

⋅

> 95% by SDS Page

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Pathogen Testing

To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.

Storage and Handling

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

2-8°C

RRID

AB_2829795

Applications and Recommended Usage?
Quality Tested by Leinco

FC The suggested concentration for this YN1/1.7.4 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
WB The suggested concentration for this YN1/1.7.4 antibody for use in western blotting is 1-10 μg/ml.

Other Applications Reported In Literature ?

CODEX®
CyTOF®
IP

Additional Reported Applications For Relevant Conjugates ?

B
IHC (Frozen)
For specific conjugates of this clone, review literature for suggested application details.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone YN1/1.7.4 recognizes an epitope on mouse CD54.

Antigen Distribution

CD54 is present on endothelial cells, lymphocytes, epithelial cells, dendritic cells and keratinocytes.

Background

ICAM-1 is a 55 kDa glycoprotein that is part of the Ig superfamily. It is heavily glycosylated to form 75 kDa to 115 kDa. ICAM-1 is known to be an adhesion and viral entry molecule, and its long suspected involevement in signal transduction is being elucidated. The signal-transducing functions of ICAM-1 appear to be mainly associated with proinflammatory pathways. Furthermore, ICAM-1 signaling appears to act as a beacon for inflammatory immune cells such as macrophages and granulocytes bringing about inflammation via lymphocyte trafficking. ICAM-1 is essential for the transmigration of leukocytes out of blood vessels and into tissues, and is a marker of endothelial dysfunction leading to damaging vascular disorders in umbilical and placental vascular tissue of gestational pregnancies. ICAM-1 is the receptor for rhinoviruses (the cause of most common colds) and malaria, and plays an inflammatory role in ocular allergies.

Antigen Details

Protein

CD54

Ligand/Receptor

CD11a/CD18 (LFA-1) or CD11b/CD18 (Mac-1) and CD11c/CD18, CD43, hyaluronan, fibrinogen

Function

Immune reaction, inflammation, adhesion

PubMed

CD54

NCBI Gene Bank ID

15894

UniProt.org

Information on Uniprot.org

Research Area

Cell Adhesion

.

Cell Biology

.

Costimulatory Molecules

.

Immunology

.

Innate Immunity

.

Neuroscience

.

Neuroscience Cell Markers

.

Stem Cell

References & Citations

1. Li, S. et al. (2009) Biochem. Biophys. Res. Commun. 381: 459 2. Wolf, S. et al. (2009) Pharmacol. Rep. 61: 22 3. Ozcan, U. et al. (2009) Arch Gynecol. Obstet.

Technical Protocols