LAG3 Antibody [2G8]

blue ice or RT

LAG-3 antibody was raised against the extracellular domain of human LAG-3

Cat. No. 1331 - Human Lymphoma Tissue Lysate

Predicted: 55 kDa

Observed: 60, 70, 80 kDa

Monoclonal

IgG1, k

LAG-3 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.

LAG-3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

None

NP_002277

3902

LAG3

Homo sapiens

The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy.

Triebel F. LAG-3: a regulator of T-cell and DC responses and its use in therapeutic vaccination. Trends Immunol 2003; 24:619–22.

Workman CJ, Vignali DA. The CD4-related molecule, LAG-3 (CD223), regulates the expansion of activated T cells. Eur J Immunol 2003; 33:970–9.

Immunocytochemistry of LAG-3 in over expressing HEK293 cells using LAG-3 antibody and control mouse IgG antibody (left corner box) at 1 μ g/ml.

Immunofluorescence of LAG-3 in over human spleen tissue using LAG-3 Antibody at 10 μ g/ml.

Red: LAG3 Antibody [2G8] (RF16082)
Blue: DAPI staining

Flow cytometry analysis of LAG-3 over expressing HEK293 cells using LAG-3 antibody at 1 μ g/ml. Blue: untransfected HEK293 cells. Yellow: LAG-3 over expressing HEK293 cells.

A sandwich ELISA was performed using the anti-LAG3 mAbs RF16082, RF16083, RF16084, RF16086, RF16087 and RF16088 as the capture antibodies for the LAG3 extracellular domain antigen with biotin-labeled Risk-Free anti-LAG3 mAbs as the detection antibodies.