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VISTA Antibody [6D2]

VISTA Antibody [6D2]

€531.00

Excl. VAT

Item no.	PRS-RF16072-0.1mg
Manufacturer	ProSci
Amount	0.1 mg
Category	Antibodies Flow Cytometry Immunohistochemistry ELISA Immunofluorescence Immunocytochemistry Primary Antibodies
Type	Antibody Monoclonal
Format	Liquid
Applications	FC, IF, ICC, ELISA, IHC-P
Specific against	Human (Homo sapiens)
Host	Mouse
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	VISTA Antibody: VISTA molecule, VSIR, B7-H5, B7H5, GI24, PP2135, SISP1, DD1alpha, VISTA, C10orf54, chromosome 10 open reading frame 54, PD-1H, V-set immunoregulatory receptor, V-Type Immunoglobulin Domain-Containing Suppressor Of T-Cell Activation, Chromo
Available
Shipping	blue ice or RT
By Research Area	Immunology
Immunogen	VISTA antibody was raised against the extracellular domain of human VISTA.
Applications	VISTA antibody can be used for immunohistochemistry starting at 5 μ g/mL and immunocytochemistry starting at 1 μ g/mL. For immunofluorescence start at 5 μ g/mL. Flow cytometry at 10 μ g/ml. Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.
Positive Control 1	Cat. No. 1331 - Human Lymphoma Tissue Lysate
Positive Control 2	Cat. No. 1306 - Human Spleen Tissue
Predicted Molecular Weight	Predicted: 31 kDa Observed: 47 kDa
Purification	VISTA Antibody is supplied as protein A purified IgG1.
Clonality	Monoclonal
Clone	6D2
Isotype	IgG1
Conjugate	Unconjugated
Buffer	VISTA Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Concentration	1 mg/mL
Storage Conditions	VISTA antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	VSIR
Accession #	NP_071436
Protein Gi #	64115
Ncbi Gene Id #	64115
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	VSIR
Ncbi Official Full Name	VISTA molecule
Ncbi Organism	Homo sapiens
Swissprot #	Q9H7M9
Background	VISTA Antibody: VISTA/B7-H5/platelet receptor Gi24 is a single-pass type I membrane protein located at the cell surface. It is an immunoregulatory receptor which can inhibit T-cell response and may promote differentiation of embryonic stem cells, by inhibiting the BMP4 signaling pathway. The protein can be cleaved by MMP14, and stimulate MMP14-mediated MMP2 activation.
Background References 1	Mayya V., et al . Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. 2009, Sci. Signal. 2:RA46-RA46.
Background References 2	Sakr M.A., et al., GI24 enhances tumor invasiveness by regulating cell surface membrane-type 1 matrix metalloproteinase. 2010, Cancer Sci. 101:2368-2374.
2nd Image Caption	Immunocytochemistry of VISTA in transfected HEK293 cells with VISTA antibody at 1 μ g/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μ g/mL.
3rd Image Caption	Immunofluorescence of VISTA in transfected HEK293 cells with VISTA antibody at 2 μ g/mL. Green: VISTA Antibody [6D2] (RF16072) Blue: DAPI staining
4th Image Caption	Immunofluorescence of VISTA in human lymphoma tissue with VISTA antibody at 10 μ g/mL. Red: VISTA Antibody [6D2] (RF16072) Blue: DAPI staining
5th Image Caption	Immunofluorescence of VISTA in human spleen tissue with VISTA antibody at 10 μ g/mL. Red: VISTA Antibody [6D2] (RF16072) Blue: DAPI staining
6th Image Caption	Immunohistochemistry of VISTA in human lymphoma tissue with VISTA antibody at 2 μ g/mL.
7th Image Caption	Flow cytometry analysis of VISTA overexpressing HEK293 cells using VISTA antibody and control mouse IgG antibody at 10 μ g/ml. Blue: Untransfected HEK293 cells. Yellow: VISTA overexpressing HEK293 cells.
8th Image Caption	Titration curve analysis of VISTA antibody to detect recombinant VISTA in ELISA at decreasing concentrations.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.1 mg

Listprice: €531.00

Price: €531.00

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Delivery expected until 9/4/2025

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