VISTA Detection Set (Risk Free)

PBS containing 0.02% sodium azide.

Antibodies can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

VISTA Antibody: VISTA/B7-H5/platelet receptor Gi24 is a single-pass type I membrane protein located at the cell surface. It is an immunoregulatory receptor which can inhibit T-cell response and may promote differentiation of embryonic stem cells, by inhibiting the BMP4 signaling pathway. The protein can be cleaved by MMP14, and stimulate MMP14-mediated MMP2 activation. ProSci's Risk-FreeTM antibodies are mouse monoclonal antibodies made to improve in vivo studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-FreeTM antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Validated for flow cytometry and ELISA Rigorously tested for the following applications: Immunoblot Immunohistochemistry Immunocytochemistry Immunofluorescence Multiple antibodies per target allowing the user to choose the best antibody for their application Available individually or as a set Risk-FreeTM means they are guaranteed to work for their approved applications

VISTA antibodies were raised against the extracellular domain of human VISTA.

Monoclonal

Mayya V., et al . Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. 2009, Sci. Signal. 2:RA46-RA46.

Cat. No. RF10001-03 - Human Spleen Tissue Lysate

Titration curve analysis of VISTA mAbs to detect recombinant LVISTA in ELISA with RF16071, RF16072, RF16073, and RF16074 abs at decreasing concentrations.

Immunocytochemistry of VISTA in over expressing HEK293 cells using (A) RF16071, (B) RF16072, (C) RF16073, (D) RF16074, and (E) control mouse IgG antibody at 1 μ g/ml.

Immunofluorescence of VISTA in human spleen tissue using (A) RF16071, (B) RF16072, (C) RF16073, (D) RF16074, and (E) control mouse IgG antibody at 10 μ g/ml.

Flow cytometry analysis of VISTA over expressing HEK293 cells using (A) RF16071, (B) RF16072, (C) RF16073, (D) RF16074, and (E) control mouse IgG antibody at 10 μ g/ml. Blue: untransfected HEK293 cells. Yellow: VISTA over expressing HEK293 cells.