LIGHT Antibody [7B9E12]

Immunology

LIGHT antibody can be used for detection of CD80 by Western blot at 0.5 - 1 μ g/mL. LIGHT antibody can be used for immunohistochemistry starting at 2 - 5 μ g/mL. For immunofluorescence start at 20 μ g/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.

Predicted: 26 kDa

Monoclonal

IgG2b

LIGHT Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.

LIGHT antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

None

NP_003798

8740

TNFSF14

Homo sapiens

LIGHT Antibody: LIGHT, also known as Tumor Necrosis Factor Superfamily member 14 (TNFSF14), is a co-stimulatory molecule that can regulate T-cell activation (1) and has recently been identified as an immune checkpoint protein. LIGHT binds to two different receptors, Herpes Virus Entry Mediator (HVEM) and Lymphotoxin beta Receptor (LTβR). While LIGHT binding to HVEM delivers a co-stimulatory signal to T cells (1), LIGHT binding to LTβR is critical for the formation of lymphoid structures which can stimulate T cell infiltration and activation of a tumor microenvironment, leading to rapid T cell-mediated tissue destruction (2). It has been shown that targeted delivery of LIGHT to tumors, thereby causing the T cell infiltration of the tumor, can enhance the response of the PD-1/PD-L1 checkpoint blockade anti-cancer therapy (3), suggesting that LIGHT may become a potent tool in anti-cancer treatment.

Lee Y, Chin RK, Christiansen P, et al. Recruitment and activation of naive T cells in the islets by lymphotoxin beta receptor-dependent tertiary lymphoid structure. Immunity 2006; 25:499-509.

Western blot analysis of LIGHT in overexpressing HEK293 cells with LIGHT antibody at 0.5 and 1 μ g/ml

Immunofluorescence of LIGHT in transfected HEK293 cells with LIGHT antibody at 2 μ g/mL.

Green: LIGHT Antibody [7B9E12] (RF16063)
Blue: DAPI staining

Immunohistochemistry of LIGHT in human lymphoma tissue with LIGHT antibody at 5 μ g/mL.