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CTLA4 Antibody [8A1]

CTLA4 Antibody [8A1]

€159.00

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Item no.	PRS-RF16013-0.02mg
Manufacturer	ProSci
Amount	0.02 mg
Category	Oncology Antibodies By Cell Type Carcinoma Immunohistochemistry Western Blot ELISA Immunofluorescence Immunocytochemistry Primary Antibodies
Type	Antibody Monoclonal
Format	Liquid
Applications	WB, IF, ICC, ELISA, IHC-P
Specific against	Human (Homo sapiens)
Host	Mouse
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	CTLA-4 Antibody: CTL4, cytotoxic T-lymphocyte associated protein 4, CD, GSE, GRD4, ALPS5, CD152, IDDM12, CELIAC3
Available
Shipping	blue ice or RT
By Research Area	Immunology
Immunogen	CTLA-4 antibody was raised against the extracellular domain of human CTLA-4.
Applications	CTLA-4 antibody can be used for detection of CTLA-4 by Western blot at 1 - 2 μ g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ g/mL. For immunofluorescence start at 20 μ g/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1	Cat. No. 1210 - HEK293 Lysate Cell Lysate
Predicted Molecular Weight	Predicted: 25 kDa Observed: 32 kDa
Purification	CTLA-4 Antibody is supplied as protein A purified IgG1.
Clonality	Monoclonal
Clone	8A1
Isotype	IgG1
Conjugate	Unconjugated
Buffer	CTLA-4 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Concentration	1 mg/mL
Storage Conditions	CTLA-4 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	CTLA4
Accession #	NP_005205
Ncbi Gene Id #	1493
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	CTLA4
Ncbi Official Full Name	cytotoxic T-lymphocyte associated protein 4
Ncbi Organism	Homo sapiens
Swissprot #	P16410
Background	The cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), also known as CD152, is a member of the immunoglobulin superfamily that is expressed by activated T cells and transmits an inhibitory signal to T cells (1, 2). Both it and the homologous T-cell co-stimulatory protein CD28 bind to CD80 (B7-H1) and CD86 (B7-H2) on antigen-presenting cells (APCs) (3). Mutations in the CTLA-4 gene have been implicated in multiple autoimmune diseases (4). CTLA-4 also functions as an immune checkpoint protein, and anti- CTLA-4 antibodies have been successfully used in the treatment of cancer (5).
Background References 1	Brunet JF, Denizot F, Luciani MF, et al. A new member of the immunoglobulin superfamily--CTLA-4 . Nature 1987; 328:267–70.
Background References 2	Walunas TL, Lenschow DJ, Bakker CY, et al. CTLA-4 can function as a negative regulator of T cell activation. Immunity 1994; 1:405–13.
Background References 3	Harding FA, McArthur JG, Gross JA, et al. CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones. Nature 1992; 356:607–9.
Background References 4	Romo-Tena J, Gomez-Martin D, and Alcocer-Verela J. CTLA-4 and autoimmunity: new insights into the dual regulator of tolerance. Autoimmun. Rev. 2013; 12:117.
1st Image Caption	Western blot analysis of CTLA-4 in overexpressing HEK293 cells CTLA-4 antibody at 0.5 and 1 μ g/mL.
2nd Image Caption	Immunocytochemistry of CTLA-4 in transfected HEK293 cells with CTLA-4 antibody at 5 μ g/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 5 μ g/mL.
3rd Image Caption	Immunofluorescence of CTLA-4 in transfected HEK293 cells with CTLA-4 antibody at 20 μ g/mL. Red: CTLA4 Antibody [8A1] (RF16013) Blue: DAPI staining
4th Image Caption	Immunofluorescence of CTLA-4 in human lymph node tissue with CTLA-4 antibody at 20 μ g/mL.
5th Image Caption	Immunofluorescence of CTLA-4 in human tonsil tissue with CTLA-4 antibody at 20 μ g/mL. Red: CTLA4 Antibody [8A1] (RF16013) Blue: DAPI staining
6th Image Caption	Immunohistochemistry of CTLA-4 in human lymph node tissue with CTLA-4 antibody at 5 μ g/mL.
7th Image Caption	Immunohistochemistry of CTLA-4 in human tonsil tissue with CTLA-4 antibody at 5 μ g/mL.
8th Image Caption	Titration curve analysis of CTLA-4 mAbs to detect recombinant CTLA-4 in ELISA with RF16011, RF16012, and RF16013 antibodies at decreasing concentrations.
9th Image Caption	A sandwich ELISA was performed using the anti-CTLA-4 mAbs RF16011, RF16012, RF16013, as the capture antibodies for the CTLA-4 extracellular domain antigen with HRP-labeled Risk-Free anti-CTLA-4 mAbs as the detection antibodies.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.02 mg

Listprice: €159.00

Price: €159.00

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Delivery expected until 10/2/2025

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