TIM-3 Antibody

blue ice or RT

TIM-3 antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of human TIM-3.

The immunogen is located within amino acids 60 - 110 of TIM-3.

At least two antibodies are known to exist; this antibody will detect both isoforms.

Predicted: 33 kDa

Observed: 37 kDa

Polyclonal

Unconjugated

1 mg/mL

Cat. No. 8659P - TIM-3 Peptide

None

NP_116171

84868

HAVCR2

Homo sapiens

The immune checkpoint protein TIM-3 is a member of the immunoglobulin superfamily and TIM family of proteins that was initially identified as a specific marker of fully differentiated IFN-γ producing CD4 T helper 1 (Th1) and CD8 cytotoxic cells. It is a Th1-specific cell surface protein that regulates macrophage activation and negatively regulates Th1-mediated auto- and alloimmune responses, and is also highly expressed on regulatory T cells, monocytes, macrophages, and dendritic cells (1). TIM-3 and PD-1 are co-expressed on most CD4 and CD8 T cells infiltrating solid tumors or in hematologic malignancy in mice; blocking TIM-3 in conjugation with a PD-1 blockade increases the functionality of exhausted T cells and synergizes with to inhibit tumor growth (2, 3).

Sakuishi K, Apetoh L, Sullivan JM, et al. Targeting Tim-3 and PD-1 pathways to reverse T cell exhaustion and restore anti-tumor immunity. J Exp Med 2010; 207:2187–94.

Western blot analysis of TIM-3 in Jurkat cell lysate with TIM-3 antibody at 1 μ g/mL in (A) the absence and (B) the presence of blocking peptide.

Immunofluorescence of TIM-3 in human spleen tissue with TIM-3 antibody at 20 μ g/ml.

Red: TIM-3 Antibody (8659)
Blue: DAPI staining