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NOX2 Antibody

NOX2 Antibody

€159.00

Excl. VAT

Item no.	PRS-7923-0.02mg
Manufacturer	ProSci
Amount	0.02 mg
Category	Antibodies Immunohistochemistry Western Blot ELISA Immunofluorescence Primary Antibodies
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IF, ELISA, IHC-P
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	NOX2 Antibody: CGD, NOX2, AMCBX2, GP91-1, GP91PHOX, p91-PHOX, GP91-PHOX, Cytochrome b-245 heavy chain, CGD91-phox, Cytochrome b558 subunit beta
Available
Shipping	blue ice or RT
By Research Area	Immunology, Neuroscience
Homology	Predicted species reactivity based on immunogen sequence: Pig: (87%), Bovine: (87%)
Immunogen	Rabbit polyclonal NOX2 antibody was raised against a 15 amino acid peptide near the amino terminus of human NOX2. The immunogen is located within amino acids 140 - 190 of NOX2.
Applications	NOX2 antibody can be used for detection of NOX2 by Western blot at 1 - 2 μ g/ml. Antibody can also be used for Immunohistochemistry starting at 2 μ g/mL. For immunofluorescence start at 20 μ g/mL. Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Specificity	NOX2 antibody is human, mouse and rat reactive. At least two isoforms are known to exist; this antibody will detect both isoforms. NOX2 is predicted to not cross-react with other NOX proteins.
Positive Control 1	Cat. No. 1463 - Rat Brain Tissue Lysate
Predicted Molecular Weight	Predicted: 59, 63 kDa Observed: 58 kDa
Purification	NOX2 antibody is affinity chromatography purified via peptide column.
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	NOX2 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Storage Conditions	NOX2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.
Related Products	Cat. No. 7923P – NOX2 Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	CYBB
Accession #	NP_000388
Protein Gi #	6996021
Ncbi Gene Id #	1536
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	CYBB
Ncbi Official Full Name	cytochrome b-245, beta polypeptide
Ncbi Organism	homo sapiens
Swissprot #	P04839
Background	The NOX family of NAPDH oxidases is comprised of seven transmembrane proteins that oxidize intracellular NAPDH/NADH, causing electron transport across the membrane and the reduction of molecular oxygen to superoxide (1). NOX2, also known as cytochrome b beta (CYBB) is one of two proteins that make up Cytochrome b-245, thought to be a primary component of the microbicidal oxidase system of phagocytes. NOX2 deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD) (2). Activation of the NOX2 enzyme complex in microglia is thought to be neurotoxic and may play a role in Alzheimer’s and Parkinson’s disease (3).
Background References 1	Bedard K and Krause KH. The Nox family of ROS-generating NAPDH oxidases: physiology and pathophysiology. Physiol. Rev. 2007; 87:245-313.
Background References 2	Segal AW. Cytochrome b-245 and its involvement in the molecular pathology of chronic granulomatous disease. Hematol. Oncol. North Am. 1988; 2:213-23.
Background References 3	Surace MJ and Block ML. Targeting microglia-mediated neurotoxicity: the potential of NOX2 inhibitors. Cell Mol. Life Sci. 2012; 69:2409-27.
1st Image Caption	Western blot analysis of NOX2 in rat brain tissue lysate with NOX2 antibody at 1 μ g/ml.
2nd Image Caption	Immunohistochemistry of NOX2 in rat brain tissue with NOX2 antibody at 5 μ g/ml.
3rd Image Caption	Immunofluorescence of NOX2 in rat brain tissue with NOX2 antibody at 20 μ g/ml.
4th Image Caption	Immunohistochemistry of NOX2 in rat brain tissue cells with NOX2 antibody at 2 μ g/ml.
5th Image Caption	Immunofluorescence of NOX2 in rat brain tissue with NOX2 antibody at 20 μ g/ml. Green: NOX2 Antibody (7923) Blue: DAPI staining

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.02 mg

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Price: €159.00

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Delivery expected until 9/4/2025

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