SPP1 Antibody

Stem Cell

SPP1 antibody was raised against an 18 amino acid peptide near the amino terminus of human SPP1.

The immunogen is located within amino acids 50 - 100 of SPP1.

SPP1 antibody is human, mouse and rat reactive. Multiple isoforms of SPP1 are known to exist.

Predicted: 36 kDa

Observed: 37 kDa

Polyclonal

Unconjugated

1 mg/mL

Cat. No. 7443P – SPP1 Peptide

None

NP_001238759

6696

SPP1

homo sapiens

SPP1 Antibody: The secreted protein 1 (SPP1), also known as osteopontin, is a major noncollagenous protein of bone, but is also found in the extracellular matrix of other mineralized tissues and in bodily fluids. In bone, SPP1 is produced by osteoblasts, osteocytes, macrophages, and osteoclasts (1, 2). SPP1 binds to cells through integrin and non-integrin receptors, as well as the adhesion receptor CD44 in an RGD-independent manner, enabling SPP1 to induce a number of functional effects including macrophage chemotaxis, cytoprotection, and regulation of T helper type 1 cells (2). SPP1 can regulate biomineralization by inhibiting the formation of hydroxyapatite (3) and the growth of calcium oxalate crystals (4).

Wang KX and Denhardt DT. Osteopontin: rule in immune regulation and stress responses. Cyto. Growth Factor Rev. 2008; 19:333-45.

Shiraga H, Min W, VanDusen WJ, et al. Inhibition of calcium oxalate crystal growth in vitro by uropontin: another member of the aspartic acid-rich protein superfamily. Proc. Natl. Acad. Sci. USA 1992; 89:426-30.

Immunohistochemistry of SPP1 in human bladder tissue with SPP1 antibody at 5 μ g/mL.