HBA2 Antibody

Predicted species reactivity based on immunogen sequence: Monkey

For FACS starting dilution is: 1:25

For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

This antibody is purified through a protein A column, followed by peptide affinity purification.

Rabbit Ig

Supplied in PBS with 0.09% (W/V) sodium azide.

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

None

P69905

3039, 3040

HBA1

Homo sapiens

HBA2 located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.

Sharma, V., et al. Hematology 14(5):297-300(2009)

Roy, P., et al. Hemoglobin 33(6):486-491(2009)

Western Blot at 1:2000 dilution + human fetal heart lysate Lysates/proteins at 20 ug per lane.

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HBA2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.