CYP3A4 Antibody

Obesity, Signal Transduction

For WB starting dilution is: 1:500

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Rabbit Ig

Supplied in PBS with 0.09% (W/V) sodium azide.

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

None

P08684

1576

CYP3A4

Homo sapiens

CYP3A4, is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs which are are used today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens.

Nelson, D.R., Pharmacogenetics 14 (1), 1-18 (2004)

Western blot analysis of lysate from human ovary tissue, using CYP3A4 Antibody at 1:1000.

Western blot analysis of CYP3A4 Antibody in NCI-H460 cell line lysates (35ug/lane)

Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.