SPHK1 Antibody

Cancer, Obesity, Signal Transduction

For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:1000

This antibody is purified through a protein A column, followed by peptide affinity purification.

Rabbit Ig

Supplied in PBS with 0.09% (W/V) sodium azide.

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

None

Q9NYA1

8877

SPHK1

Homo sapiens

Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.

Antibody staining SPHK1 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Western Blot at 1:2000 dilution Lane 1: human liver lysate Lane 2: HUVEC whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of lysates from 293, HepG2, HUVEC, Raji, rat C6, mouse C2C12 cell line (from left to right), using SPHK1 Antibody (R301) at 1:1000 at each lane.

Western blot analysis of anti-SPHK1 Antibody in 293 cell line lysates (35ug/lane)