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Pyruvate Kinase Antibody

Pyruvate Kinase Antibody

€645.00

Excl. VAT

Item no.	PRS-62-617-200ul
Manufacturer	ProSci
Amount	200 ul
Category	Oncology Metabolism Glucose Homeostasis Antibodies Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies By Organ Thyroid Cancer Immunostaining
Type	Antibody Primary
Format	Liquid
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Pyruvate kinase PKM, Cytosolic thyroid hormone-binding protein, CTHBP, Opa-interacting protein 3, OIP-3, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1, THBP1, Tumor M2-PK, p58, PKM, OIP3, PK2, PK3, PKM2
Available
Shipping	blue ice or RT
By Research Area	Cancer, Obesity, Signal Transduction
Immunogen	This Pyruvate Kinase (PKM2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-505 amino acids from the C-terminal region of human Pyruvate Kinase (PKM2).
Applications	For WB starting dilution is: 1:1000 For IF starting dilution is: 1:200 For IHC-P starting dilution is: 1:50~1:100
Predicted Molecular Weight	58 kDa
Purification	This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality	Polyclonal
Isotype	Rabbit Ig
Conjugate	Unconjugated
Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration	batch dependent
Storage Conditions	Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	PKM
Accession #	P14618
Protein Gi #	20178296
Ncbi Gene Id #	5315
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	PKM
Ncbi Official Full Name	Pyruvate kinase PKM
Ncbi Organism	Homo sapiens
Swissprot #	P14618
Background	There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis.
Background References 1	References for protein:
Background References 2	Williams, J.M., et al., Mol. Microbiol. 27(1):171-186 (1998).
Background References 3	Gress, T.M., et al., Oncogene 13(8):1819-1830 (1996).
Background References 4	Kato, H., et al., Proc. Natl. Acad. Sci. U.S.A. 86(20):7861-7865 (1989).
1st Image Caption	Western blot analysis of lysates from HeLa, rat PC-12, Ramos, mouse NIH/3T3 cell line, mouse brain tissue lysate(from left to right), using PKM2-N491 at 1:1000 at each lane.
2nd Image Caption	Western blot analysis of lysates from HeLa, rat PC-12, Ramos, mouse NIH/3T3 cell line, mouse brain tissue lysate(from left to right), using PKM2-N491 at 1:1000 at each lane.
3rd Image Caption	Fluorescent confocal image of MCF7 cells stained with Pyruvate Kinase (PKM2) antibody. MCF7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with Pyruvate Kinase (PKM2) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the Pyruvate Kinase (PKM2) mainly to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000067225).
4th Image Caption	Western blot analysis of anti-h PKM2-N491 Pab in Ramos cell line lysates (35ug/lane).
5th Image Caption	Pyruvate Kinase (PKM2) Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Pyruvate Kinase (PKM2) Antibody for immunohistochemistry.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 200 ul

Listprice: €645.00

Price: €645.00

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Delivery expected until 8/7/2025

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