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Vimentin Antibody

Vimentin Antibody

€645.00

Excl. VAT

Item no.	PRS-61-470-400ul
Manufacturer	ProSci
Amount	400 ul
Category	Oncology Neuroscience Neural Lineage Markers Molecular Targets for Neuroscience Neural Development Antibodies Cell Biology Cytoskeleton Flow Cytometry Immunohistochemistry Western Blot Primary Antibodies Intermediate filaments Stem Cell Markers
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, FC, IHC-P
Specific against	Human (Homo sapiens)
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Vimentin, VIM
Available
Shipping	blue ice or RT
By Research Area	Cancer, Cell Cycle, Neuroscience, Signal Transduction
Homology	Predicted species reactivity based on immunogen sequence: Bovine, Hamster, Mouse, Pig, Rat
Immunogen	This Vimentin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-181 amino acids from the Central region of human Vimentin.
Applications	For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50
Predicted Molecular Weight	54 kDa
Purification	This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality	Polyclonal
Isotype	Rabbit Ig
Conjugate	Unconjugated
Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration	batch dependent
Storage Conditions	Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	VIM
Accession #	P08670
Protein Gi #	55977767
Ncbi Gene Id #	7431
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	VIM
Ncbi Official Full Name	Vimentin
Ncbi Organism	Homo sapiens
Swissprot #	P08670
Background	Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.
Background References 1	Whipple, R.A., Cancer Res. 68 (14), 5678-5688 (2008)
Background References 2	Garcia-Verdugo, I., Biochemistry 47 (18), 5127-5138 (2008)
Background References 3	Merdes, A., J. Cell Biol. 115 (2), 397-410 (1991)
1st Image Caption	Western blot analysis of Vimentin Antibody in A2058, A375 cell line lysates (35ug/lane)
2nd Image Caption	Western blot analysis of Vimentin using Vimentin Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VIM gene (Lane 2).
3rd Image Caption	Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Vimentin antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
4th Image Caption	Vimentin Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
5th Image Caption	Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 400 ul

Listprice: €645.00

Price: €645.00

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Delivery expected until 10/2/2025

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