Connexin 40 Antibody

Signal Transduction

For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Rabbit Ig

Supplied in PBS with 0.09% (W/V) sodium azide.

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

None

P36382

2702

GJA5

Homo sapiens

Gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell closely packed transmembrane channels. Proteins, called connexins, purified from fractions of enriched gap junctions from different tissues differ. Connexins are designated by their molecular mass. Another system of nomenclature divides gap junction proteins into 2 categories, alpha and beta, according to sequence similarities at the nucleotide and amino acid levels. For example, CX43 is designated alpha-1 gap junction protein, whereas CX32 and CX26 are called beta-1 and beta-2 gap junction proteins, respectively. This nomenclature emphasizes that CX32 and CX26 are more homologous to each other than either of them is to CX43. Connexins have four transmembrane, three intracellular, and two extracellular regions. Different tissues express different connexins, though tissue specificities overlap, and a given tissue or cell can express several different connexins. Developmental regulation of at least some of the connexin genes has been found. Embryo implantation is regulated in part by temporally changing patterns of expression of connexins in the embryo and the maternal decidua.

Simon, A.M., et al., Dev. Biol. 251(2):206-220 (2002).

Oviedo-Orta, E., et al., Immunology 99(4):578-590 (2000).

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GJA5 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.