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FADS2 Antibody

FADS2 Antibody

€645.00

Excl. VAT

Item no.	PRS-56-329-200ul
Manufacturer	ProSci
Amount	200 ul
Category	Antibodies Flow Cytometry Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies Immunostaining
Type	Antibody Primary
Format	Liquid
Applications	WB, FC, IF, IHC-P
Specific against	Human (Homo sapiens)
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Fatty acid desaturase 2, 11419-, Delta(6) fatty acid desaturase, D6D, Delta(6) desaturase, Delta-6 desaturase, FADS2
Available
Shipping	blue ice or RT
By Research Area	Obesity, Neuroscience, Signal Transduction
Homology	Predicted species reactivity based on immunogen sequence: Monkey
Immunogen	This FADS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 79-108 amino acids from the N-terminal region of human FADS2.
Applications	For IF starting dilution is: 1:10~50 For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50
Predicted Molecular Weight	52 kDa
Purification	This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality	Polyclonal
Isotype	Rabbit Ig
Conjugate	Unconjugated
Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration	batch dependent
Storage Conditions	Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	FADS2
Accession #	O95864
Ncbi Gene Id #	9415
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	FADS2
Ncbi Official Full Name	Fatty acid desaturase 2
Ncbi Organism	Homo sapiens
Swissprot #	O95864
Background	The protein encoded by this gene is a member of the fattyacid desaturase (FADS) gene family. Desaturase enzymes regulateunsaturation of fatty acids through the introduction of doublebonds between defined carbons of the fatty acyl chain. FADS familymembers are considered fusion products composed of an N-terminalcytochrome b5-like domain and a C-terminal multiplemembrane-spanning desaturase portion, both of which arecharacterized by conserved histidine motifs. This gene is clusteredwith family members FADS1 and FADS2 at 11q12-q13.1; this cluster isthought to have arisen evolutionarily from gene duplication basedon its similar exon/intron organization.
Background References 1	Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background References 2	Mathias, R.A., et al. J. Lipid Res. 51(9):2766-2774(2010)
Background References 3	Lu, Y., et al. Am. J. Clin. Nutr. 92(1):258-265(2010)
Background References 4	Zabaneh, D., et al. PLoS ONE(8), E11961 (2010) :
1st Image Caption	Fluorescent image of A549 cell stained with FADS2 Antibody (N-term). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FADS2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).FADS2 immunoreactivity is localized to Cytoplasm and Vesicles significantly.
2nd Image Caption	Western blot analysis in HepG2 cell line lysates (35ug/lane).
3rd Image Caption	FADS2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
4th Image Caption	Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 200 ul

Listprice: €645.00

Price: €645.00

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Delivery expected until 8/7/2025

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