SCF Antibody

Stem Cell

Anti-SCF antibody (5165) was raised against a peptide corresponding to 18 amino acids near the center of human SCF.

The immunogen is located within amino acids 100-150 of SCF.

Cat. No. 1202 – A431 Cell Lysate

Predicted: 31kD

Observed: 36 kD (31kD + 4 N-linked Glycosylations)

Human SCF has 3 isoforms, including isoform 1 (273aa, 30.9kD), isoform 2 (245aa, 27.9kD) and isoform 3 (238aa, 26.7kD). Mouse SCF has 2 isoforms, including isoform 1 (273aa, 30.6kD) and isoform 2 (245aa, 27.6kD). Rat SCF also has 2 isoforms, including isoform 1 (273aa, 30.7kD) and isoform 2 (245aa, 27.7kD). 5165 can detect human and possibly detect mouse and rat.

Polyclonal

Unconjugated

1 mg/mL

Cat. No. 5165P – SCF Peptide

None

P21583

4254

KITLG

Homo sapiens

SCF Antibody: Stem cell factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways. Binding of SCF by the c-Kit receptor leads to homodimerization of the receptor and the activation of signalling pathways such as PI-3, PLC-gamma, Jak/STAT, and MAP kinase pathways. SCF expression leads to the induction of mast cell survival and the expression and release of histamine, pro-inflammatory cytokines and chemokines. The inhibition of the SCF/c-Kit pathway leads to a decrease in histamine levels, mast cell and eosinophil infiltration, IL-4 production and airway hyperresponsiveness, suggesting this pathway may be a useful therapeutic target in inflammatory diseases such as asthma. At least two isoforms of SCF are known to exist.

Jensen et al. Inflamm. Allergy Drug Targets 2007; 6:57-62.

Zhang et al. Up-regulation of the Ang II/AT1 receptor may compensate for the loss of gastric antrum ICC via the PI3k/Akt signaling pathway in STZ-induced diabetic mice. Mol Cell Endocrinol. 2016; 423:77-86.PMID: 26773730

Figure 1 Western Blot Validation in Cell Lines and Tissues of Human, Mouse and Rat
Loading: 15 μ g of lysates per lane.Antibodies: SCF 5165 (1 μ g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 3 Western Blot Validation in Human HeLa Cell Lines
Loading: 15 μ g of lysates per lane.Antibodies: SCF 5165 (2 μ g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 5 Immunofluorescence Validation of SCF in Human Brain Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labeling SCF with 5165 at 20 μ g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Figure 7 Regulated Expression Validation of SCF in Gastric Smooth Muscle Cells (GSMCs) of Mice (Zhang et al., 2016)
WB analysis of protein expression level of SCF detected by anti-SCF antibody (5165). GSMCs were treated with PI3K inhibitor (LY294002), AT1R inhibitor (ZD7155) and AT2R inhibitor (PD123319) before Ang II treatment. Ang II (10^-8mol/L) significantly increased SCF protein expression, which was reduced by treatment of LY294002 and ZD7155.