AP-2 gamma Antibody

blue ice or RT

Expected Species Reactivity based on sequence homology: Rat, Mouse, Dog, Pig, Cow

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:WB experiments gave bands at approx 75kDa and 22kDa in Human Placenta and Ovary lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 49.2kDa according to NP_003213.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Skin shows nuclear staining of keratinocytes. Recommended concentration, 2-3ug/ml.

Cat. No. 1376 - Human Skin Tissue Lysate

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Unconjugated

500 ug/mL

Cat. No. 45-259P - AP-2 gamma Peptide

None

NP_003213.1

7022

transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma)

Q92754

45-259 (4ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.