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FOXP3 Antibody

FOXP3 Antibody

€645.00

Excl. VAT

Item no.	PRS-45-055-0.1mg
Manufacturer	ProSci
Amount	0.1 mg
Category	Antibodies Immunohistochemistry Western Blot ELISA Immunocytochemistry Primary Antibodies CHiP
Type	Antibody Primary
Format	Liquid
Applications	WB, FC, IF, ICC, ELISA
Specific against	Human (Homo sapiens)
Host	Goat
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	FOXP3, SCURFIN, forkhead box P3, JM2, AIID, IPEX, PIDX, XPID, DIETER, JM2 protein, immunodeficiency, polyendocrinopathy, enteropathy, X-linked, immune dysregulation, polyendocrinopathy, enteropathy, X-linked, MGC141961, MGC141963, FOXP3delta7, scurfin
Available
Shipping	blue ice or RT
By Research Area	Signal Transduction
Immunogen	The immunogen for this antibody is: SQRPSRCSNPTPGP
Applications	Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 48-50kDa band observed in Human Muscle lysates and in lysates of cell line MOLT4 (calculated MW of 47.2kDa according to NP_054728.2). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Pancreas lysate.Immunofluorescence: Strong expression of the protein seen in the nucleoplasm of U2OS cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml. Immunocytochemistry: CD25-sorted (Treg) Human blood cells with nuclear speckled staining. Recommended concentration 2-4ug/ml for overnight staining.
Specificity	This antibody is expected to recognize both reported isoforms (NP_054728.2 and NP_001107849.1)
Positive Control 1	Cat. No. 1315 - Human Tonsil Tissue Lysate
Positive Control 2	Cat. No. 11-533 - Human Tonsil Tissue Slide
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality	Polyclonal
Conjugate	Unconjugated
Buffer	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration	500 ug/mL
Storage Conditions	Aliquot and store at -20˚ C. Minimize freezing and thawing.
Related Products	Cat. No. 45-055P - FOXP3 Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	FOXP3
Accession #	NP_054728.2, NP_001107849.1
Protein Gi #	31982943
Ncbi Gene Id #	50943
Ncbi Official Symbol	FOXP3
Ncbi Official Full Name	forkhead box P3
Ncbi Organism	Homo sapiens
Swissprot #	Q9BZS1
Background References 1	Chatila TA, Blaeser F, Ho N, Lederman HM, Voulgaropoulos C, Helms C, Bowcock AM. JM2, encoding a fork head-related protein, is mutated in X-linked autoimmunity-allergic disregulation syndrome. J Clin Invest. 2000 Dec; 106(12):R75-81.
1st Image Caption	45-055 (1ug/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Nuclear staining of scattered cells in the interfollicular area. B) High magnification of positive cells. This d
2nd Image Caption	45-055 staining of CD25-sorted (Treg) Human blood cells gathered by cytospin and detected by FITC (A) and in phase contrast (B).
3rd Image Caption	45-055 (1ug/ml) staining of Human Muscle (A), (2ug/ml) MOLT4 (B) and (1ug/ml) negative control Pancreas (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
4th Image Caption	45-055 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing strong localization to nucleoplasm. The nuclear st
5th Image Caption	45-055 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml). IgG control: Unimmunized goat IgG (black line) f

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.1 mg

Listprice: €645.00

Price: €645.00

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Delivery expected until 9/4/2025

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