Comparison

Hdac1 Antibody

€645.00
Excl. VAT
Item no. PRS-43-003-0.1mg
Manufacturer ProSci
Amount 0.1 mg
Category
Type Antibody Primary
Format Liquid
Applications WB, ELISA, CHIP
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus)
Host Goat
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias HDAC1, histone deacetylase 1, RP23-209C6.7, HD1, Hdac1-ps, MommeD5, RPD3, MGC102534, MGC118085, OTTMUSP00000010171
Available
Shipping
blue ice or RT
Homology
Expected Species Reactivity based on sequence homology: Rat, Pig, Cow
Immunogen
The immunogen for this antibody is: C-PEDAIPEESGDE
Applications
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 55kDa band observed in lysates of cell line NIH3T3 (calculated MW of 55.1kDa according to Human NP_032254.1 and Mouse NP_032254.1). Recommended concentration: 0.3-1ug/ml.Chromatin Immunoprecipitation: A 4-fold increased presence of H3 onto the Human P21 locus observed when comparing the precipitations using EB11428 with non-specific goat IgG to bring down HDAC1 in a chromatin lysate from MCF7 cells.
Specificity
This antibody may cross-react to HDAC2
Positive Control 1
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Predicted Molecular Weight
Approx 55 kDa
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Conjugate
Unconjugated
Buffer
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration
500 ug/mL
Storage Conditions
Aliquot and store at -20˚ C. Minimize freezing and thawing.
Related Products
Cat. No. 43-003P - Hdac1 Peptide
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
Hdac1
Accession #
NP_032254.1
Protein Gi #
6680193
Ncbi Gene Id #
3065
Ncbi Official Symbol
Hdac1
Ncbi Official Full Name
histone deacetylase 1
Ncbi Organism
Homo sapiens
Swissprot #
Q13547
1st Image Caption
43-003 (0.3ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
2nd Image Caption
ChIP of 2ug 43-003 with MCF7 chromatin using the Chromatrap® spin column sonication kit (Protein G) measuring H3 enrichment onto the p21 locus.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.1 mg
Available: In stock
Listprice: €645.00
Price: €645.00
available

Delivery expected until 9/4/2025 

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